The project aims to set the stage for a new era of virtual 3D histology using contrast-enhanced microfocus computed tomography (CECT) by developing and validating novel contrast-enhancing staining agents (CESAs). A multidisciplinary approach is applied, crossing the boundaries between biology, engineering, imaging and chemical synthesis. We will develop and synthesize novel CESAs that specifically stain different components of the extracellular matrix in whole tissues. One example within this project is the development of antigen-specific CESAs, which are comparable to immunohistochemistry. Hence, we propose ex-vivo high-resolution CE-CT imaging to become a non-invasive quantitative 3D anatomical tool that will allow unprecedented 3D characterization of the biological tissues.