Journal papers

Maes, A., Borgel, O., Braconnier, C., Balcaen, T., Wevers, M., Halbgebauer, R., … & Kerckhofs, G. (2024). X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT. International Journal of Biomedical Imaging2024.

Xie, M., Kaiser, M., Gershtein, Y., Schnyder, D., Deviatiiarov, R., Gazizova, G., … & Chagin, A. S. (2024). The level of protein in the maternal murine diet modulates the facial appearance of the offspring via mTORC1 signaling. Nature Communications15(1), 1-15.

Schröer, L., Balcaen, T., Folens, K., Boon, N., De Kock, T., Kerckhofs, G., & Cnudde, V. (2024). 3D Visualization of Cyanobacterial Biofilms using Micro-Computed Tomography with Contrast-Enhancing Staining Agents. Tomography of Materials and Structures, 100024.

Davis, S., Karali, A., Balcaen, T., Zekonyte, J., Pétré, M., Roldo, M., Kerckhofs, G. & Blunn, G. (2024). Comparison of two contrast-enhancing staining agents for use in X-ray imaging and digital volume correlation measurements across the cartilage-bone interface. Journal of the Mechanical Behavior of Biomedical Materials, 106414.

Pestiaux, C.; Marino, A.; Simal, L.; Horman, S.; Capoulade, R.; Kerckhofs, G. X-ray-Based 3D Histology of Murine Hearts Using Contrast-Enhanced Microfocus Computed Tomography (CECT) and Cryo-CECT. Hearts 2024, 5, 14-28.

Benova A, Ferencakova M, Bardova K, Funda J, Prochazka J, Spoutil F, Cajka T, Dzubanova M, Balcaen T, Kerckhofs G, Willekens W, van Lenthe GH, Charyyeva A, Alquicer G, Pecinova A, Mracek T, Horakova O, Coupeau R, Hansen MS, Rossmeisl M, Kopecky J, Tencerova M. Omega-3 PUFAs prevent bone impairment and bone marrow adiposity in mouse model of obesity. Commun Biol. 2023 Oct 14;6(1):1043. doi: 10.1038/s42003-023-05407-8.

Leyssens, L., Balcaen, T., Pétré, M., Ayllón, N. B., El Aazmani, W., de Pierpont, A., … & Kerckhofs, G. Non-destructive 3D characterization of the blood vessel wall microstructure in different species and blood vessel types using contrast-enhanced microCT and comparison with synthetic vascular grafts. Acta Biomaterialia164, 303-316 (2023).

Dehkord, E.S., Kerckhofs, G., Compere, P., Lambert, F., Geris, L. with Geris, L. (corresp. author) (2023). An Empirical Model Linking Physico-Chemical Biomaterial Characteristics to Intra-Oral Bone Formation. JOURNAL OF FUNCTIONAL BIOMATERIALS, 14 (7), Art.No. ARTN 388. doi: 10.3390/jfb14070388

Pestiaux, C., Pyka, G., Quirynen, L., De Azevedo, D., Vanoverschelde, J. L., Lengelé, B., … & Kerckhofs, G. 3D histopathology of stenotic aortic valve cusps using ex vivo microfocus computed tomography. Frontiers in Cardiovascular Medicine10, 1129990 (2023).

Salmon, P., Monzem, S., Javaheri, B., Oste, L., Kerckhofs, G., & Pitsillides, A. A. Resolving trabecular metaphyseal bone profiles downstream of the growth plate adds value to bone histomorphometry in mouse models. Frontiers in Endocrinology14, 839 (2023).

Balcaen, T., Piens, C., Mwema, A., Chourrout, M., Vandebroek, L., Rieux, A.D., Chauveau, F., De Borggraeve, W.M., Hoffmann, D. and Kerckhofs, G., Revealing the three-dimensional murine brain microstructure by contrast-enhanced computed tomography. Frontiers in Neuroscience17, p.465 (2023).

Frangi, G., Guicheteau, M., Jacquot, F., Pyka, G., Kerckhofs, G., Feyeux, M., … & Beck-Cormier, S. PiT2 deficiency prevents increase of bone marrow adipose tissue during skeletal maturation but not in OVX-induced osteoporosis. Frontiers in Endocrinology, 2864 (2022).

Starovoyt, A., Quirk, B.C., Putzeys, T. et al. An optically-guided cochlear implant sheath for real-time monitoring of electrode insertion into the human cochlea. Sci Rep 12, 19234 (2022).

Starovoyt, A., Shaheen, E., Putzeys, T., Kerckhofs, G., Politis, C., Wouters, J., Verhaert, N. with Verhaert, N.(corresp. author) (2023). Anatomically and mechanically accurate scala tympani model for electrode insertion studies. HEARING RESEARCH, 430, Art.No. ARTN 108707. doi: 10.1016/j.heares.2023.108707

Starovoyt, A., Pyka, G., Putzeys, T., Balcaen, T., Wouters, J., Kerckhofs, G., Verhaert, N. with Verhaert, N.(corresp. author) (2023). Human cochlear microstructures at risk of electrode insertion trauma, elucidated in 3D with contrast-enhanced microCT. SCIENTIFIC REPORTS, 13 (1), Art.No. ARTN 2191. doi: 10.1038/s41598-023-29401-6

Lang, A., Benn, A., Wolter, A., Balcaen, T., Collins, J., Kerckhofs, G., Zwijsen, A., Boerckel, J.D. (2023). Endothelial SMAD1/5 signaling couples angiogenesis to osteogenesis during long bone growth. bioRxiv. doi: 10.1101/2023.01.07.522994

Maes, A., Pestiaux, C., Marino, A., Balcaen, T., Leyssens, L., Vangrunderbeeck, S., Pyka, G., De Borggraeve W.M., Bertrand, L., Beauloye, C., Horman, S., Wevers, M., Kerckhofs, G.  Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues. Nat Commun 13, 6207 (2022).

Ali, D. A. H., Figeac, F., Tencerova, M., Caci, A., Ditzel, N., Schmal, C., Kerckhofs, G., Havelund, J., Færgeman, N., Rauch, A., Tencerova, M., and, Kassem, M. High fat diet (HFD)-induced obesity augments the deleterious effects of estrogen deficiency in bone. Evidence from post-menopausal mice. Bone Reports13(Suppl.), P155 (2022).

Benova, A., Ferencakova, M., Bardova, K., Funda, J., Prochazka, J., Spoutil, F., Cajka, T., Dzubanova, M., Balcaen, T., Kerckhofs, G., Willekens, W., van Lenthe, H., Alquicer, G., Pecinova, A., Mracek, T., Horakova, O., Rossmeisl, M., Kopecky, J. and, Tencerova, M. Novel thiazolidinedione analog reduces a negative impact on bone and mesenchymal stem cell properties in obese mice compared to classical thiazolidinediones. Molecular Metabolism, p.101598 (2022).

Léger, J., Leyssens, L., Kerckhofs, G., De Vleeschouwer, C. Ensemble learning and test-time augmentation for the segmentation of mineralized cartilage versus bone in high-resolution microCT images. Computers in Biology and Medicine, 105932 (2022).

Pascart, T., Falgayrac, G., Cortet, B., Paccou, J., Bleuse, M., Coursier, R., … & Budzik, J. F. Subchondral involvement in osteonecrosis of the femoral head: insight on local composition, microstructure and vascularization. Osteoarthritis and Cartilage (2022).

Figeac, F., Tencerova, M., Ali, D., Andersen, T.L., Appadoo, D.R C., Kerckhofs, G., Ditzel, N., Kowal, J.M., Rauch, A., Kassem, M. (2022). Impaired bone fracture healing in type 2 diabetes is caused by defective functions of skeletal progenitor cells. Stem Cells, Art.No. sxab011. doi: 10.1093/stmcls/sxab011

Tam, W.L., Mendes, L.F., Chen, X., Lesage, R., Van Hoven, I., Leysen, E., Kerckhofs, G., Bosmans, K., Chai, Y.C., Yamashita, A., Tsumaki, N., Geris, L., Roberts, S.J., Luyten, F.P. with Luyten, F.P. (corresp. author) (2021). Human pluripotent stem cell-derived cartilaginous organoids promote scaffold-free healing of critical size long bone defects. STEM CELL RESEARCH & THERAPY, 12 (1), Art.No. ARTN 513. doi: 10.1186/s13287-021-02580-7

Marin, C., Tuts, J., Luyten, F., Vandamme, K., Kerckhofs, G. Impaired soft and hard callus formation during fracture healing in diet-induced obese mice as revealed by 3D contrast-enhanced computed tomography imaging. Bone, Volume 150 (2021).

Leyssens, L., Pestiaux, C., Kerckhofs, G. A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System. Int. J. Mol. Sci.22, 3263 (2021).

de Bournonville, S., Geris, L. & Kerckhofs, G. Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state. Sci Rep 11, 2819 (2021).

Colsoul, N., Marin, C., Corbeels, K., Kerckhofs, G., Van der Schueren, B., Vandamme, K. with Vandamme, K.(corresp. author) (2020). Alteration of the Condylar Oral Bone in Obese and Gastric Bypass Mice. CALCIFIED TISSUE INTERNATIONAL, 107 (4), 371-380. doi: 10.1007/s00223-020-00732-0

de Bournonville, S., Vangrunderbeeck, S., Ly, H. G. T., Geeroms, C., De Borggraeve, W. M., Parac-Vogt, T. N., & Kerckhofs, G., Exploring Polyoxometalates as Non-Destructive Staining Agents for Contrast-Enhanced Microfocus Computed Tomography of Biological Tissues. Acta Biomaterialia (2020).

Tratwal, J., Labella, R., Bravenboer, N., Kerckhofs, G., Douni, E., Scheller, E.L., Badr, S., Karampinos, D.C., BeckCournier, S., Palmisano, B., Poloni, A., Moreno-Aliaga, M.J., Fretz, J., Rodeheffer, M.S., Boroumand, P., Rosen, C.J., Horowitz, M.C., van der Eerden, B.C J., Veldhuis-Vlug, A.G., Naveiras, O. (2020). Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society. Frontiers In Endocrinology, 11, Art.No. ARTN 65. doi: 10.3389/fendo.2020.00065

Pascart, T., Paccou, J., Colard, T., Norberciak, L., Girard, J., Delattre, J., Marchandise, P., Legrand, J., Penel, G., Coursier, R., Putman, S., Cortet, B., Kerckhofs, G., Budzik, J-F. (2020). T1-weighted MRI images accurately represent the volume and surface of architectural mineral damage of osteonecrosis of the femoral head: Comparison with high-resolution computed tomography. Bone, 130, Art.No. ARTN 115099. doi: 10.1016/j.bone.2019.115099

Kamperman, T., Moreira Teixeira, L., Salehi, S.S., Kerckhofs, G., Guyot, Y., Geven, M., Geris, L., Grijpma, D., Blanquer, S., Leijten, J. (2020). Engineering 3D parallelized microfluidic droplet generators with equal flow profiles by computational fluid dynamics and stereolithographic printing. Lab On A Chip, 20 (3), 490-495. doi: 10.1039/c9lc00980a

Beekman, K.M., Zwaagstra, M., Veldhuis-Vlug, A.G., van Essen, H.W., den Heijer, M., Maas, M., Kerckhofs, G., ParacVogt, T.N., Bisschop, P.H., Bravenboer, N. with Bravenboer, N. (corresp. author) (2019). Ovariectomy increases RANKL protein expression in bone marrow adipocytes of C3H/HeJ mice. AMERICAN JOURNAL OF PHYSIOLOGYENDOCRINOLOGY AND METABOLISM, 317 (6), E1050-E1054. doi: 10.1152/ajpendo.00142.2019

Penel, G., Kerckhofs, G., Chauveau, C. with Chauveau, C. (corresp. author) (2019). Brief Report From the 4th International Meeting on Bone Marrow Adiposity (BMA2018). Frontiers In Endocrinology, 10, Art.No. 691, 1-5. doi: 10.3389/fendo.2019.00691

Zhao, L., Ding, L., Soete, J., Idrissi, H., Kerckhofs, G., Simar, A. with Zhao, L. (corresp. author) (2019). Fostering crack deviation via local internal stresses in Al/NiTi composites and its correlation with fracture toughness. Composites Part A-Applied Science And Manufacturing, 126. doi: 10.1016/j.compositesa.2019.105617

Coutel, X., Delattre, J., Marchandise, P., Falgayrac, G., Béhal, H., Kerckhofs, G., Penel, G., Olejnik, C. with Olejnik, C. (corresp. author) (2019). Mandibular bone is protected against microarchitectural alterations and bone marrow adipose conversion in ovariectomized rats. BONE, 127, 343-352. doi: 10.1016/j.bone.2019.06.031

de Bournonville S., Vangrunderbeeck S., Kerckhofs G.,Contrast-Enhanced MicroCT for Virtual 3D Anatomical Pathology of Biological Tissues: A Literature Review. Contrast media & molecular imaging 2019 (2019).

De Clercq, K., Persoons, E., Napso, T., Luyten, C., Parac-Vogt, T., Sferruzzi-Perri A., Kerckhofs G., Vriens. J., High-resolution contrast-enhanced microCT reveals the true three-dimensional morphology of the murine placenta. Proceedings of the National Academy of Sciences (2019): 201902688.

Bolette, A., Lecloux, G., Rompen, E., Albert, A., Kerckhofs, G., Lambert, F. (2019). Influence of induced infection in medication-related osteonecrosis of the jaw development after tooth extraction: A study in rats. Journal Of Cranio-Maxillofacial Surgery, 47 (2), 349-356. doi: 10.1016/j.jcms.2018.08.011

Beekman, K.M., Veldhuis-Vlug, A.G., van der Veen, A., den Heijer, M., Maas, M., Kerckhofs, G., Parac-Vogt, T.N., Bisschop, P.H., Bravenboer, N. (2019). The effect of PPAR gamma inhibition on bone marrow adipose tissue and bone in C3H/HeJ mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 316 (1), E96-E105. doi: 10.1152/ajpendo.00265.2018

Ji, W., Kerckhofs, G., Geeroms, C., Marechal, M., Geris, L., Luyten, F.P. with Luyten, F.P. (corresp. author) (2018). Deciphering the combined effect of bone morphogenetic protein 6 and calcium phosphate on bone formation capacity of periosteum derived cells-based tissue engineering constructs. ACTA BIOMATERIALIA, 80, 97-107. doi: 10.1016/j.actbio.2018.09.046

Cao, W., Sun, T., Kerckhofs, G., Fardell, G., Price, B., Dewulf, W. with Cao, W. (corresp. author) (2018). A simulation-based study on the influence of the x-ray spectrum on the performance of multi-material beam hardening correction algorithms. MEASUREMENT SCIENCE AND TECHNOLOGY, 29 (9), Art.No. ARTN 095002. doi: 10.1088/1361-6501/aacba0

Marin, C., Papantonakis, G., Sels, K., van Lenthe, H., Falgayrac, G., Vangoitsenhoven, R., Van Der Schueren, B., Penel, G., Luyten, F., Vandamme, K., Kerckhofs, G. (2018). Unraveling the compromised biomechanical performance of type 2 diabetes- and Roux-en-Y gastric bypass bone by linking mechanical-structural and physico-chemical properties. Scientific Reports, 8, Art.No. 5881, 5881-1. doi: 10.1038/s41598-018-24229-x

Kerckhofs, G., Stegen, S., van Gastel, N., Sap, A., Falgayrac, G., Penel, G., Durand, M., Luyten, F., Geris, L., Vandamme, K., Parac-Vogt, T., Carmeliet, G. (2018). Simultaneous three-dimensional visualization of mineralized and soft skeletal tissues by a novel microCT contrast agent with polyoxometalate structure. Biomaterials, 159, 1-12. doi: 10.1016/j.biomaterials.2017.12.016

Marin, C., Luyten, F., Van Der Schueren, B., Kerckhofs, G., Vandamme, K. (2018). The impact of type 2 diabetes on bone fracture healing. Frontiers in Endocrinology, 9 (6), Art.No. 6, 1-15. doi: 10.3389/fendo.2018.00006

Coutel, X., Olejnik, C., Marchandise, P., Delattre, J., Béhal, H., Kerckhofs, G., Penel, G. (2018). A novel microCT method for bone and marrow adipose tissue alignment identifies key differences between mandible and tibia in rats. Calcified Tissue International, 103, 189-197. doi: 10.1007/s00223-018-0397-1